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dapi fluorescence intensities fis  (BMG Labtech)


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    BMG Labtech dapi fluorescence intensities fis
    Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green <t>fluorescence</t> intensities (FIs) were assessed in living mDANs. CellRox and <t>DAPI</t> FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)
    Dapi Fluorescence Intensities Fis, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 98/100, based on 7799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi fluorescence intensities fis/product/BMG Labtech
    Average 98 stars, based on 7799 article reviews
    dapi fluorescence intensities fis - by Bioz Stars, 2026-06
    98/100 stars

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    1) Product Images from "NPT100-18A rescues mitochondrial oxidative stress and neuronal degeneration in human iPSC-based Parkinson's model."

    Article Title: NPT100-18A rescues mitochondrial oxidative stress and neuronal degeneration in human iPSC-based Parkinson's model.

    Journal: BMC neuroscience

    doi: 10.1186/s12868-025-00926-y

    Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green fluorescence intensities (FIs) were assessed in living mDANs. CellRox and DAPI FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)
    Figure Legend Snippet: Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green fluorescence intensities (FIs) were assessed in living mDANs. CellRox and DAPI FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)

    Techniques Used: Derivative Assay, Control, Inhibition, Activation Assay, Fluorescence, Microscopy, Luciferase, Staining, Cell Culture



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    dapi fluorescence intensities fis  (BMG Labtech)
    98
    BMG Labtech dapi fluorescence intensities fis
    Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green <t>fluorescence</t> intensities (FIs) were assessed in living mDANs. CellRox and <t>DAPI</t> FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)
    Dapi Fluorescence Intensities Fis, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi fluorescence intensities fis/product/BMG Labtech
    Average 98 stars, based on 1 article reviews
    dapi fluorescence intensities fis - by Bioz Stars, 2026-06
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green fluorescence intensities (FIs) were assessed in living mDANs. CellRox and DAPI FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)

    Journal: BMC neuroscience

    Article Title: NPT100-18A rescues mitochondrial oxidative stress and neuronal degeneration in human iPSC-based Parkinson's model.

    doi: 10.1186/s12868-025-00926-y

    Figure Lengend Snippet: Fig. 1 Increased oxidative stress and mitochondrial dysfunction in patient-derived mDANs. (A) Schematic of experimental design. Fibroblasts from PD patients carrying a monoallelic SNCA locus duplication and unaffected control individuals were obtained through punch biopsy of the skin. Fibroblasts were reprogrammed into human iPSCs. Human iPSCs were first differentiated into NPCs through dual SMAD inhibition and activation of canonical Wnt signaling and then into mDANs using a FGF8b-based protocol. mDANs were treated with NPT100-18A during the entire differentiation and maturation periods. (B-D) Relative levels depicted as fold changes from the mean of control cell line C1 ± SD of n wells from N independent differentiations. Repli cates belonging to the same differentiation are shown in the same color. (B) CellRox Green fluorescence intensities (FIs) were assessed in living mDANs. CellRox and DAPI FIs were measured at 80 sites per well in 96-well plates using a CLARIOstar plate reader. CellRox FIs were normalized to respective DAPI FIs. Dots representing single well means (n = 15, N = 4). Right panels show representative microscopy images of CellRox and DAPI fluorescence signals. (C) Significantly reduced relative ATP levels measured in patient-derived compared to control mDANs. Relative ATP levels were assessed in mDAN lysates using a luciferase-based assay and a LUMIstar Omega plate reader. Values for single wells were normalized to the frequency of viable neurons (determined with a viability staining in mDANs cultured in parallel under the same conditions). Dots represent single wells (n = 9, N = 3). (D) MitoSOX Red FIs were measured in living mDANs analogously to (B). Dots represent single well means (n = 18, N = 4). Right panels show representative microscopy images of MitoSOX and DAPI fluorescence signals; for better visualization of neurons, β3-tubulin (TUBB3) was additionally immunofluorescently labelled. (B-D) Student’s t-test, *P < 0.05, ** P < 0.01, ***P < 0.001. Scale bar 50 μm in (B) and 20 μm in (D)

    Article Snippet: CellROX, MitoSOX, and DAPI fluorescence intensities (FIs) were measured on a CLARIOstar Plus microplate reader (BMG Labtech) using the following excitation/emission wavelengths: CellROX – 500 − 20/530 − 25 nm; MitoSOX – 510 − 15/580 − 20 nm; DAPI – 360 − 20/460 − 30 nm.

    Techniques: Derivative Assay, Control, Inhibition, Activation Assay, Fluorescence, Microscopy, Luciferase, Staining, Cell Culture